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  • br Conflict of interest statement br The authors have

    2020-08-03


    Conflict of interest statement
    The authors have no conflicts of interests with other persons or organizations.
    Acknowledgments
    This study Elafibranor (GFT505) sponsored in part by three research grants as follows: HR15-016 from Oklahoma Center for the Advancement of Science and Technology (OCAST), Grant R01 CA197150 from the National Cancer Institute, National Institutes of Health, USA, and SCC research award from Stephenson Cancer Center at the University of Oklahoma Health Sciences Center.
    References
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    Contents lists available at ScienceDirect
    International Journal of Biological Macromolecules
    Aptamer functionalized curcumin-loaded human serum albumin (HSA) nanoparticles for targeted delivery to HER-2 positive breast cancer cells
    Tayebeh Saleh, Tooba Soudi, Seyed Abbas Shojaosadati
    Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, PO Box: 14155-114, Iran
    Article history:
    Keywords:
    Human serum albumin nanoparticle (HSA NP) Curcumin
    Human epithelial growth factor receptor 2
    Aptamer
    Active targeting 
    In this study, an HER2 aptamer-decorated curcumin-loaded human serum albumin nanoparticle (Apt-HSA/CCM NP) was developed and characterized as a new anticancer formulation for targeted delivery to human epithelial growth factor receptor 2 (HER2) overexpressing breast cancer cells. Conjugation of HER2 Apt to the surface of HSA NPs was confirmed by gel electrophoresis and FTIR analysis. The obtained NPs have the hydrodynamic diam-eter of 281.1 ± 11.1 nm and zeta potential of −33.3 ± 2.5 mV. The data demonstrated that encapsulation of curcumin in HSA NPs by desolvation method has increased water solubility by 400 folds. Fluorescent microscopy image demonstrated remarkable cytoplasmic uptake of Apt-HSA/CCM NPs in HER2-overexpressing SK-BR-3 cells compared to unconjugated counterparts. Cytotoxicity experiments demonstrated no significant difference be-tween cytotoxic effect of free curcumin and non-targeted HSA/CCM NPs in both HER2 positive and HER2 negative cell lines. However, the toxicity of Apt-HSA/CCM NPs was significantly higher and cell viability reached 36% after 72 h in SK-BR3 cell line. These results suggest that this targeted delivery system has the potential to be considered as a promising candidate for the treatment of HER2 positive cancer cells.